An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The midnolin-proteasome pathway catches proteins for ubiquitination-independent degradation.

Gu X, Nardone C, Kamitaki N, Mao A, Elledge SJ, Greenberg ME

Cells use ubiquitin to mark proteins for proteasomal degradation. Although the proteasome also eliminates proteins that are not ubiquitinated, how this occurs mechanistically is unclear. Here, we found that midnolin promoted the destruction of many nuclear proteins, including transcription factors encoded by the immediate-early genes. Diverse stimuli induced midnolin, and its overexpression was sufficient to cause the degradation of its ... [more]

Science Aug. 25, 2023; 381(6660);eadh5021 [Pubmed: 37616343]

Throughput

High Throughput

Additional Notes

HA-tagged Midnolin expressed in HEK-293T cells treated with DMSO.
HA-tagged Midnolin expressed in HEK-293T cells treated with the proteasome inhibitor MG132.

Curated By

BioGRID

Interaction Summary

MIDN

KPNA2

Gene Ontology Biological Process

Gene Ontology Molecular Function

histone deacetylase binding [IPI]nuclear localization sequence binding [IDA]poly(A) RNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

Affinity Capture-MS

Publication

The midnolin-proteasome pathway catches proteins for ubiquitination-independent degradation.

Throughput

Additional Notes

Curated By

histone deacetylase binding [IPI]
nuclear localization sequence binding [IDA]
poly(A) RNA binding [IDA]
protein binding [IPI]