PREY
PCBP2
HNRNPE2, HNRPE2, hnRNP-E2
poly(rC) binding protein 2
GO Process (8)
GO Function (5)
GO Component (7)
Gene Ontology Biological Process
- RNA splicing [TAS]
- gene expression [TAS]
- innate immune response [TAS]
- mRNA metabolic process [NAS]
- mRNA splicing, via spliceosome [TAS]
- negative regulation of defense response to virus [IMP]
- negative regulation of type I interferon production [TAS]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The midnolin-proteasome pathway catches proteins for ubiquitination-independent degradation.
Cells use ubiquitin to mark proteins for proteasomal degradation. Although the proteasome also eliminates proteins that are not ubiquitinated, how this occurs mechanistically is unclear. Here, we found that midnolin promoted the destruction of many nuclear proteins, including transcription factors encoded by the immediate-early genes. Diverse stimuli induced midnolin, and its overexpression was sufficient to cause the degradation of its ... [more]
Science Aug. 25, 2023; 381(6660);eadh5021 [Pubmed: 37616343]
Throughput
- High Throughput
Additional Notes
- HA-tagged Midnolin expressed in HEK-293T cells treated with DMSO.
- HA-tagged Midnolin expressed in HEK-293T cells treated with the proteasome inhibitor MG132.
Curated By
- BioGRID