An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The midnolin-proteasome pathway catches proteins for ubiquitination-independent degradation.

Gu X, Nardone C, Kamitaki N, Mao A, Elledge SJ, Greenberg ME

Cells use ubiquitin to mark proteins for proteasomal degradation. Although the proteasome also eliminates proteins that are not ubiquitinated, how this occurs mechanistically is unclear. Here, we found that midnolin promoted the destruction of many nuclear proteins, including transcription factors encoded by the immediate-early genes. Diverse stimuli induced midnolin, and its overexpression was sufficient to cause the degradation of its ... [more]

Science Aug. 25, 2023; 381(6660);eadh5021 [Pubmed: 37616343]

Throughput

High Throughput

Additional Notes

HA-tagged Midnolin expressed in HEK-293T cells treated with DMSO.
HA-tagged Midnolin expressed in HEK-293T cells treated with the proteasome inhibitor MG132.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ELAVL1 MIDN	Affinity Capture-RNA Affinity Capture-RNA An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.	Abdelmohsen K (2009)	High	-	BioGRID	664138

Curated By

BioGRID

Interaction Summary

MIDN

ELAVL1

Gene Ontology Biological Process

Gene Ontology Molecular Function

AU-rich element binding [IDA]RNA binding [IDA]double-stranded RNA binding [IDA]mRNA 3'-UTR AU-rich region binding [IDA]mRNA 3'-UTR binding [ISS]mRNA binding [TAS]poly(A) RNA binding [IDA]protein binding [IPI]protein kinase binding [IPI]

Gene Ontology Cellular Component

Affinity Capture-MS