PREY
RAN
ARA24, Gsp1, TC4, OK/SW-cl.81
RAN, member RAS oncogene family
GO Process (15)
GO Function (7)
GO Component (9)
Gene Ontology Biological Process
- DNA metabolic process [TAS]
- androgen receptor signaling pathway [NAS]
- gene expression [TAS]
- intracellular transport of virus [TAS]
- mitotic nuclear division [TAS]
- mitotic spindle organization [TAS]
- positive regulation of protein binding [IDA]
- positive regulation of transcription, DNA-templated [NAS]
- protein export from nucleus [IDA]
- ribosomal large subunit export from nucleus [IMP]
- ribosomal small subunit export from nucleus [IMP]
- signal transduction [TAS]
- small molecule metabolic process [TAS]
- viral life cycle [TAS]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The midnolin-proteasome pathway catches proteins for ubiquitination-independent degradation.
Cells use ubiquitin to mark proteins for proteasomal degradation. Although the proteasome also eliminates proteins that are not ubiquitinated, how this occurs mechanistically is unclear. Here, we found that midnolin promoted the destruction of many nuclear proteins, including transcription factors encoded by the immediate-early genes. Diverse stimuli induced midnolin, and its overexpression was sufficient to cause the degradation of its ... [more]
Science Aug. 25, 2023; 381(6660);eadh5021 [Pubmed: 37616343]
Throughput
- High Throughput
Additional Notes
- HA-tagged Midnolin expressed in HEK-293T cells treated with DMSO.
- HA-tagged Midnolin expressed in HEK-293T cells treated with the proteasome inhibitor MG132.
Curated By
- BioGRID