BAIT

MIDN

midnolin
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY

FUS

ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS
FUS RNA binding protein
GO Process (3)
GO Function (4)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The midnolin-proteasome pathway catches proteins for ubiquitination-independent degradation.

Gu X, Nardone C, Kamitaki N, Mao A, Elledge SJ, Greenberg ME

Cells use ubiquitin to mark proteins for proteasomal degradation. Although the proteasome also eliminates proteins that are not ubiquitinated, how this occurs mechanistically is unclear. Here, we found that midnolin promoted the destruction of many nuclear proteins, including transcription factors encoded by the immediate-early genes. Diverse stimuli induced midnolin, and its overexpression was sufficient to cause the degradation of its ... [more]

Science Aug. 25, 2023; 381(6660);eadh5021 [Pubmed: 37616343]

Throughput

  • High Throughput

Additional Notes

  • HA-tagged Midnolin expressed in HEK-293T cells treated with DMSO.
  • HA-tagged Midnolin expressed in HEK-293T cells treated with the proteasome inhibitor MG132.

Curated By

  • BioGRID