Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

O-GlcNAcylation of SPOP promotes carcinogenesis in hepatocellular carcinoma.

Zhou P, Chang WY, Gong DA, Huang LY, Liu R, Liu Y, Xia J, Wang K, Tang N, Huang AL

Aberrantly elevated O-GlcNAcylation level is commonly observed in human cancer patients, and has been proposed as a potential therapeutic target. Speckle-type POZ protein (SPOP), an important substrate adaptor of cullin3-RING ubiquitin ligase, plays a key role in the initiation and development of various cancers. However, the regulatory mechanisms governing SPOP and its function during hepatocellular carcinoma (HCC) progression remain unclear. ... [more]

Oncogene Mar. 01, 2023; 42(10);725-736 [Pubmed: 36604567]

Throughput

  • Low Throughput

Curated By

  • BioGRID