BAIT

DCAF12

CT102, KIAA1892, TCC52, WDR40A
DDB1 and CUL4 associated factor 12
UPS Project
GO Process (1)
GO Function (0)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens
PREY

CCT5

CCT-epsilon, CCTE, HEL-S-69, TCP-1-epsilon
chaperonin containing TCP1, subunit 5 (epsilon)
Homo sapiens

Biochemical Activity (Ubiquitination)

An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

Recognition of the CCT5 di-Glu degron by CRL4DCAF12 is dependent on TRiC assembly.

Pla-Prats C, Cavadini S, Kempf G, Thomae NH

Assembly Quality Control (AQC) E3 ubiquitin ligases target incomplete or incorrectly assembled protein complexes for degradation. The CUL4-RBX1-DDB1-DCAF12 (CRL4DCAF12 ) E3 ligase preferentially ubiquitinates proteins that carry a C-terminal double glutamate (di-Glu) motif. Reported CRL4DCAF12 di-Glu-containing substrates include CCT5, a subunit of the TRiC chaperonin. How DCAF12 engages its substrates and the functional relationship between CRL4DCAF12 and CCT5/TRiC is currently ... [more]

EMBO J Feb. 15, 2023; 42(4);e112253 [Pubmed: 36715408]

Throughput

  • Low Throughput

Additional Notes

  • The reaction contained UBA1 [E1], UBE2L3 [E2], and CUL4A, CUL4B, RBX1, DDB1, DCAF12 [E3]

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CCT5 DCAF12
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9999BioGRID
3083931
DCAF12 CCT5
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
3568746

Curated By

  • BioGRID