PREY

PDE6D

JBTS22, PDED
phosphodiesterase 6D, cGMP-specific, rod, delta
GO Process (1)
GO Function (3)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Palmitoylation and PDE6? regulate membrane-compartment-specific substrate ubiquitylation and degradation.

Liang D, Jiang L, Bhat SA, Missiroli S, Perrone M, Lauriola A, Adhikari R, Gudur A, Vasi Z, Ahearn I, Guardavaccaro D, Giorgi C, Philips M, Kuchay S

Substrate degradation by the ubiquitin proteasome system (UPS) in specific membrane compartments remains elusive. Here, we show that the interplay of two lipid modifications and PDE6? regulates compartmental substrate targeting via the SCFFBXL2. FBXL2 is palmitoylated in a prenylation-dependent manner on cysteines 417 and 419 juxtaposed to the CaaX motif. Palmitoylation/depalmitoylation regulates its subcellular trafficking for substrate engagement and degradation. ... [more]

Cell Rep Jan. 31, 2023; 42(1);111999 [Pubmed: 36662618]

Throughput

  • Low Throughput

Curated By

  • BioGRID