PREY

PARK2

AR-JP, LPRS2, PDJ, PRKN, KB-152G3.1
parkin RBR E3 ubiquitin protein ligase
GO Process (58)
GO Function (22)
GO Component (12)

Gene Ontology Biological Process

Homo sapiens

FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Publication

Identification of the ubiquitin-like domain of midnolin as a new glucokinase interaction partner.

Hofmeister-Brix A, Kollmann K, Langer S, Schultz J, Lenzen S, Baltrusch S

Glucokinase acts as a glucose sensor in pancreatic beta cells. Its posttranslational regulation is important but not yet fully understood. Therefore, a pancreatic islet yeast two-hybrid library was produced and searched for glucokinase-binding proteins. A protein sequence containing a full-length ubiquitin-like domain was identified to interact with glucokinase. Mammalian two-hybrid and fluorescence resonance energy transfer analyses confirmed the interaction between ... [more]

J. Biol. Chem. Dec. 13, 2013; 288(50);35824-39 [Pubmed: 24187134]

Throughput

  • High Throughput

Curated By

  • BioGRID