ESYT1
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ARL6IP5
Gene Ontology Biological Process
- L-glutamate transport [ISS]
- intrinsic apoptotic signaling pathway in response to oxidative stress [IDA]
- negative regulation of mitochondrial membrane potential [IMP]
- positive regulation of apoptotic process [IMP]
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- positive regulation of stress-activated MAPK cascade [IMP]
Gene Ontology Cellular Component
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
ESYT1 tethers the ER to mitochondria and is required for mitochondrial lipid and calcium homeostasis.
Mitochondria interact with the ER at structurally and functionally specialized membrane contact sites known as mitochondria-ER contact sites (MERCs). Combining proximity labelling (BioID), co-immunoprecipitation, confocal microscopy and subcellular fractionation, we found that the ER resident SMP-domain protein ESYT1 was enriched at MERCs, where it forms a complex with the outer mitochondrial membrane protein SYNJ2BP. BioID analyses using ER-targeted, outer mitochondrial ... [more]
Throughput
- High Throughput
Additional Notes
- BioID
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
ESYT1 ARL6IP5 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 1066866 |
Curated By
- BioGRID