Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

ESYT1 tethers the ER to mitochondria and is required for mitochondrial lipid and calcium homeostasis.

Janer A, Morris JL, Krols M, Antonicka H, Aaltonen MJ, Lin ZY, Anand H, Gingras AC, Prudent J, Shoubridge EA

Mitochondria interact with the ER at structurally and functionally specialized membrane contact sites known as mitochondria-ER contact sites (MERCs). Combining proximity labelling (BioID), co-immunoprecipitation, confocal microscopy and subcellular fractionation, we found that the ER resident SMP-domain protein ESYT1 was enriched at MERCs, where it forms a complex with the outer mitochondrial membrane protein SYNJ2BP. BioID analyses using ER-targeted, outer mitochondrial ... [more]

Life Sci Alliance Jan. 01, 2024; 7(1); [Pubmed: 37931956]

Throughput

  • High Throughput

Additional Notes

  • BioID

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
OCLN ESYT2
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High38.62BioGRID
2997916

Curated By

  • BioGRID