SPT6
Gene Ontology Biological Process
- chromatin maintenance [IMP]
- chromatin organization involved in regulation of transcription [IGI, IMP]
- chromatin remodeling [IGI, IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription from RNA polymerase II promoter by glucose [IMP]
- nucleosome assembly [IDA]
- nucleosome organization [IMP]
- poly(A)+ mRNA export from nucleus [IMP]
- positive regulation of transcription elongation from RNA polymerase II promoter [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of transcription involved in G1/S transition of mitotic cell cycle [IMP]
- regulation of histone H3-K36 methylation [IDA, IMP]
- regulation of mRNA 3'-end processing [IGI, IMP]
- regulation of nucleosome density [IMP]
- regulation of transcription from RNA polymerase II promoter in response to stress [IMP]
- regulation of transcriptional start site selection at RNA polymerase II promoter [IMP]
- transcription antitermination [IGI, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
POB3
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The social and structural architecture of the yeast protein interactome.
Cellular functions are mediated by protein-protein interactions, and mapping the interactome provides fundamental insights into biological systems. Affinity purification coupled to mass spectrometry is an ideal tool for such mapping, but it has been difficult to identify low copy number complexes, membrane complexes and complexes that are disrupted by protein tagging. As a result, our current knowledge of the interactome ... [more]
Quantitative Score
- 6.0 [Score_FDR+correlation]
Throughput
- High Throughput
Additional Notes
- Protein interactions were identified using statistically significant enrichment of the proteins in the forward and reverse pull-downs, as well as making use of the profile similarities of interacting proteins in a correlation analysis. High confidence interactions have a total score >=2. This score is a sum of the FDR score of the forward pull-down + FDR score of the reverse pull-down + correlation score.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SPT6 POB3 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| POB3 SPT6 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | Low | - | BioGRID | - | |
| SPT6 POB3 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.1238 | BioGRID | 1934919 | |
| POB3 SPT6 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | Low | - | BioGRID | 157269 | |
| SPT6 POB3 | Synthetic Rescue Synthetic Rescue A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene. | High | - | BioGRID | 3312432 |
Curated By
- BioGRID