SEC61
Gene Ontology Biological Process
- ER-associated ubiquitin-dependent protein catabolic process [IMP, IPI]
- SRP-dependent cotranslational protein targeting to membrane, translocation [IDA]
- intracellular protein transmembrane import [IMP]
- misfolded protein transport [IMP]
- posttranslational protein targeting to membrane, translocation [IDA, IMP]
- retrograde protein transport, ER to cytosol [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
KAR2
Gene Ontology Biological Process
- ER-associated ubiquitin-dependent protein catabolic process [IMP]
- SRP-dependent cotranslational protein targeting to membrane, translocation [IMP]
- karyogamy involved in conjugation with cellular fusion [IGI, IMP]
- posttranslational protein targeting to membrane, translocation [IDA, IMP]
- response to unfolded protein [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The social and structural architecture of the yeast protein interactome.
Cellular functions are mediated by protein-protein interactions, and mapping the interactome provides fundamental insights into biological systems. Affinity purification coupled to mass spectrometry is an ideal tool for such mapping, but it has been difficult to identify low copy number complexes, membrane complexes and complexes that are disrupted by protein tagging. As a result, our current knowledge of the interactome ... [more]
Quantitative Score
- 4.0 [Score_FDR+correlation]
Throughput
- High Throughput
Additional Notes
- Protein interactions were identified using statistically significant enrichment of the proteins in the forward and reverse pull-downs, as well as making use of the profile similarities of interacting proteins in a correlation analysis. High confidence interactions have a total score >=2. This score is a sum of the FDR score of the forward pull-down + FDR score of the reverse pull-down + correlation score.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| KAR2 SEC61 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.2481 | BioGRID | 1938834 | |
| SEC61 KAR2 | PCA PCA A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay. | Low | - | BioGRID | 430004 |
Curated By
- BioGRID