BAIT
WTM1
L000003515, YOR230W
Transcriptional modulator; involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats
GO Process (6)
GO Function (2)
GO Component (1)
Gene Ontology Biological Process
- maintenance of protein location in nucleus [IMP]
- negative regulation of chromatin silencing at silent mating-type cassette [IGI]
- negative regulation of chromatin silencing at telomere [IGI]
- negative regulation of transcription from RNA polymerase II promoter during meiosis [IGI]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, IMP]
- protein import into nucleus [IMP, IPI]
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
PREY
CCT7
TCP7, L000002763, YJL111W
Subunit of the cytosolic chaperonin Cct ring complex; related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance
GO Process (1)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The social and structural architecture of the yeast protein interactome.
Cellular functions are mediated by protein-protein interactions, and mapping the interactome provides fundamental insights into biological systems. Affinity purification coupled to mass spectrometry is an ideal tool for such mapping, but it has been difficult to identify low copy number complexes, membrane complexes and complexes that are disrupted by protein tagging. As a result, our current knowledge of the interactome ... [more]
Nature Nov. 15, 2023; (); [Pubmed: 37968396]
Quantitative Score
- 4.0 [Score_FDR+correlation]
Throughput
- High Throughput
Additional Notes
- Protein interactions were identified using statistically significant enrichment of the proteins in the forward and reverse pull-downs, as well as making use of the profile similarities of interacting proteins in a correlation analysis. High confidence interactions have a total score >=2. This score is a sum of the FDR score of the forward pull-down + FDR score of the reverse pull-down + correlation score.
Curated By
- BioGRID