BAIT

SBA1

CST18, L000004284, L000002999, S000029122, YKL117W

Co-chaperone that binds and regulates Hsp90 family chaperones; plays a role in determining prion variants; important for pp60v-src activity in yeast; homologous to the mammalian p23 proteins, and like p23 can regulate telomerase activity; protein abundance increases in response to DNA replication stress

GO Process (4)

GO Function (1)

GO Component (2)

Gene Ontology Biological Process

negative regulation of DNA binding [IDA]

positive regulation of telomere maintenance via telomerase [IDA, IMP]

protein folding [IMP, IPI]

regulation of telomerase activity [IDA, IMP]

Gene Ontology Molecular Function

chaperone binding [IMP, IPI]

Gene Ontology Cellular Component

cytoplasm [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

DGR2

YKL121W

Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds; DGR2 has a paralog, YMR102C, that arose from the whole genome duplication

GO Process (0)

GO Function (0)

GO Component (0)

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The social and structural architecture of the yeast protein interactome.

Michaelis AC, Brunner AD, Zwiebel M, Meier F, Strauss MT, Bludau I, Mann M

Cellular functions are mediated by protein-protein interactions, and mapping the interactome provides fundamental insights into biological systems. Affinity purification coupled to mass spectrometry is an ideal tool for such mapping, but it has been difficult to identify low copy number complexes, membrane complexes and complexes that are disrupted by protein tagging. As a result, our current knowledge of the interactome ... [more]

Nature Nov. 15, 2023; (); [Pubmed: 37968396]

Quantitative Score

2.0 [Score_FDR+correlation]

Throughput

High Throughput

Additional Notes

Protein interactions were identified using statistically significant enrichment of the proteins in the forward and reverse pull-downs, as well as making use of the profile similarities of interacting proteins in a correlation analysis. High confidence interactions have a total score >=2. This score is a sum of the FDR score of the forward pull-down + FDR score of the reverse pull-down + correlation score.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SBA1 DGR2	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Echtenkamp FJ (2011)	High	-	BioGRID	-
SBA1 DGR2	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Echtenkamp FJ (2011)	High	-	BioGRID	557010
SBA1 DGR2	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Echtenkamp FJ (2011)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary