SRB2
Gene Ontology Biological Process
- RNA polymerase II transcriptional preinitiation complex assembly [IDA]
- negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to chemical stimulus [IMP]
- negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to nutrient levels [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
Gene Ontology Molecular Function- RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly [IDA]
- RNA polymerase II transcription factor recruiting transcription factor activity [IMP]
- TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA]
- core RNA polymerase II binding transcription factor activity [IDA, IGI]
- protein domain specific binding [IGI]
- RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly [IDA]
- RNA polymerase II transcription factor recruiting transcription factor activity [IMP]
- TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA]
- core RNA polymerase II binding transcription factor activity [IDA, IGI]
- protein domain specific binding [IGI]
Gene Ontology Cellular Component
GAL11
Gene Ontology Biological Process
- RNA polymerase II transcriptional preinitiation complex assembly [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of invasive growth in response to glucose limitation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
Gene Ontology Molecular Function- RNA polymerase II activating transcription factor binding [IDA]
- RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly [IDA]
- RNA polymerase II transcription factor recruiting transcription factor activity [IMP]
- TFIIE-class binding transcription factor activity [IDA]
- TFIIE-class transcription factor binding [IDA]
- TFIIH-class transcription factor binding [IDA]
- RNA polymerase II activating transcription factor binding [IDA]
- RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly [IDA]
- RNA polymerase II transcription factor recruiting transcription factor activity [IMP]
- TFIIE-class binding transcription factor activity [IDA]
- TFIIE-class transcription factor binding [IDA]
- TFIIH-class transcription factor binding [IDA]
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The social and structural architecture of the yeast protein interactome.
Cellular functions are mediated by protein-protein interactions, and mapping the interactome provides fundamental insights into biological systems. Affinity purification coupled to mass spectrometry is an ideal tool for such mapping, but it has been difficult to identify low copy number complexes, membrane complexes and complexes that are disrupted by protein tagging. As a result, our current knowledge of the interactome ... [more]
Quantitative Score
- 5.0 [Score_FDR+correlation]
Throughput
- High Throughput
Additional Notes
- Protein interactions were identified using statistically significant enrichment of the proteins in the forward and reverse pull-downs, as well as making use of the profile similarities of interacting proteins in a correlation analysis. High confidence interactions have a total score >=2. This score is a sum of the FDR score of the forward pull-down + FDR score of the reverse pull-down + correlation score.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GAL11 SRB2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| GAL11 SRB2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| GAL11 SRB2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| SRB2 GAL11 | Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps. | Low | - | BioGRID | - | |
| GAL11 SRB2 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -7.4404 | BioGRID | 215429 |
Curated By
- BioGRID