BAIT

SAE2

COM1, ssDNA endodeoxyribonuclease SAE2, L000002892, YGL175C
Endonuclease required for telomere elongation; also required for telomeric 5' C-rich strand resection; involved in processing hairpin DNA structures with MRX complex; involved in double-strand break repair; required for normal resistance to DNA-damaging agents; exists in form of inactive oligomers that are transiently released into smaller active units by a series of phosphorylations; DNA damage triggers removal of Sae2p ensuring that active Sae2p is present only transiently
Saccharomyces cerevisiae (S288c)
PREY

POL3

CDC2, HPR6, TEX1, DNA-directed DNA polymerase delta POL3, L000000242, YDL102W
Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)
Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Double-strand breaks induce inverted duplication chromosome rearrangements by a DNA polymerase ?-dependent mechanism.

Al-Zain AM, Nester MR, Ahmed I, Symington LS

Inverted duplications, also known as foldback inversions, are commonly observed in cancers and are the major class of chromosome rearrangement recovered from yeast cells lacking Mre11 nuclease activity. Foldback priming at DNA double-strand breaks (DSBs) is one mechanism proposed for the generation of inverted duplications. However, the other pathway steps have not been fully elucidated. Here, we show that a ... [more]

Nat Commun Nov. 02, 2023; 14(1);7020 [Pubmed: 37919272]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: chromosome/plasmid maintenance (APO:0000143)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SAE2 POL3
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2222BioGRID
2436448
POL3 SAE2
Phenotypic Suppression
Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
872466

Curated By

  • BioGRID