Protein-peptide

An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.

Publication

Large-scale phosphomimetic screening identifies phospho-modulated motif-based protein interactions.

Kliche J, Garvanska DH, Simonetti L, Badgujar D, Dobritzsch D, Nilsson J, Davey NE, Ivarsson Y

Phosphorylation is a ubiquitous post-translation modification that regulates protein function by promoting, inhibiting or modulating protein-protein interactions. Hundreds of thousands of phosphosites have been identified but the vast majority have not been functionally characterised and it remains a challenge to decipher phosphorylation events modulating interactions. We generated a phosphomimetic proteomic peptide-phage display library to screen for phosphosites that modulate short linear ... [more]

Mol Syst Biol Jul. 11, 2023; 19(7);e11164 [Pubmed: 37219487]

Throughput

  • High Throughput

Additional Notes

  • Phage display

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
YAP1 HNRNPUL1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
2473859

Curated By

  • BioGRID