BAIT

ELOF1

ELF1
elongation factor 1 homolog (S. cerevisiae)
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY

SACS

ARSACS, DNAJC29, PPP1R138, SPAX6
sacsin molecular chaperone
GO Process (2)
GO Function (3)
GO Component (6)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

ELOF1 is a transcription-coupled DNA repair factor that directs RNA polymerase II ubiquitylation.

van der Weegen Y, de Lint K, van den Heuvel D, Nakazawa Y, Mevissen TET, van Schie JJM, San Martin Alonso M, Boer DEC, Gonzalez-Prieto R, Narayanan IV, Klaassen NHM, Wondergem AP, Roohollahi K, Dorsman JC, Hara Y, Vertegaal ACO, de Lange J, Walter JC, Noordermeer SM, Ljungman M, Ogi T, Wolthuis RMF, Luijsterburg MS

Cells employ transcription-coupled repair (TCR) to eliminate transcription-blocking DNA lesions. DNA damage-induced binding of the TCR-specific repair factor CSB to RNA polymerase II (RNAPII) triggers RNAPII ubiquitylation of a single lysine (K1268) by the CRL4CSA ubiquitin ligase. How CRL4CSA is specifically directed towards K1268 is unknown. Here, we identify ELOF1 as the missing link that facilitates RNAPII ubiquitylation, a key ... [more]

Nat Cell Biol Dec. 01, 2020; 23(6);595-607 [Pubmed: 34108663]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: growth abnormality (HP:0001507) [viability (PATO:0000169)]

Additional Notes

  • CRISPR GI screen
  • Cell Line: RPE1-iCas9
  • Experimental Setup: Timecourse
  • GIST: A-phenotypic negative genetic interaction
  • Library: TKOv3
  • Significance Threshold: p<0.001

Curated By

  • BioGRID