Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Evolutionarily conserved regulators of tau identify targets for new therapies.

Kim J, de Haro M, Al-Ramahi I, Garaicoechea LL, Jeong HH, Sonn JY, Tadros B, Liu Z, Botas J, Zoghbi HY

Tauopathies are neurodegenerative diseases that involve the pathological accumulation of tau proteins; in this family are Alzheimer disease, corticobasal degeneration, and chronic traumatic encephalopathy, among others. Hypothesizing that reducing this accumulation could mitigate pathogenesis, we performed a cross-species genetic screen targeting 6,600 potentially druggable genes in human cells and Drosophila. We found and validated 83 hits in cells and further ... [more]

Neuron Mar. 15, 2023; 111(6);824-838.e7 [Pubmed: 36610398]

Quantitative Score

  • 16.22 [Confidence Score]

Throughput

  • High Throughput

Additional Notes

  • Affinity capture MS was carried out to identify high confidence protein interactors
  • log2(Fold, Target/Control) reported

Curated By

  • BioGRID