An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Antagonistic roles of canonical and Alternative-RPA in disease-associated tandem CAG repeat instability.

Gall-Duncan T, Luo J, Jurkovic CM, Fischer LA, Fujita K, Deshmukh AL, Harding RJ, Tran S, Mehkary M, Li V, Leib DE, Chen R, Tanaka H, Mason AG, Levesque D, Khan M, Razzaghi M, Prasolava T, Lanni S, Sato N, Caron MC, Panigrahi GB, Wang P, Lau R, Castel AL, Masson JY, Tippett L, Turner C, Spies M, La Spada AR, Campos EI, Curtis MA, Boisvert FM, Faull RLM, Davidson BL, Nakamori M, Okazawa H, Wold MS, Pearson CE

Expansions of repeat DNA tracts cause >70 diseases, and ongoing expansions in brains exacerbate disease. During expansion mutations, single-stranded DNAs (ssDNAs) form slipped-DNAs. We find the ssDNA-binding complexes canonical replication protein A (RPA1, RPA2, and RPA3) and Alternative-RPA (RPA1, RPA3, and primate-specific RPA4) are upregulated in Huntington disease and spinocerebellar ataxia type 1 (SCA1) patient brains. Protein interactomes of RPA ... [more]

Cell Oct. 26, 2023; 186(22);4898-4919.e25 [Pubmed: 37827155]

Throughput

High Throughput

Additional Notes

>5 log2-fold enrichment and p value < 0.01 versus untransfected controls
BioID
HU treated HEK293T cells (25.2396 log2-fold enrichment)
Untreated HEK293T cells (23.0762 log2-fold enrichment)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
BRIP1 RPA1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Gupta R (2007)	Low	-	BioGRID	-
RPA1 BRIP1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Gupta R (2007)	Low	-	BioGRID	-
BRIP1 RPA1	Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.	Gong Z (2010)	Low	-	BioGRID	-
RPA1 BRIP1	Far Western Far Western An interaction is detected between a protein immobilized on a membrane and a purified protein probe.	Gupta R (2007)	Low	-	BioGRID	-
RPA1 BRIP1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Gupta R (2007)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

RPA1

Gene Ontology Biological Process

Gene Ontology Molecular Function

damaged DNA binding [IDA]protein binding [IPI]single-stranded DNA binding [IDA]

Gene Ontology Cellular Component

BRIP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP-dependent DNA helicase activity [NAS]DNA binding [NAS]protein binding [IPI]