BAIT

FYN

SLK, SYN, p59-FYN, RP1-66H14.1
FYN proto-oncogene, Src family tyrosine kinase
GO Process (29)
GO Function (6)
GO Component (5)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Fyn Regulates Binding Partners of Cyclic-AMP Dependent Protein Kinase A.

Schmoker AM, Barritt SA, Weir ME, Mann JE, Hogan TC, Ballif BA, Deming PB

The cAMP-dependent protein kinase A (PKA) is a serine/threonine kinase involved in many fundamental cellular processes, including migration and proliferation. Recently, we found that the Src family kinase Fyn phosphorylates the catalytic subunit of PKA (PKA-C) at Y69, thereby increasing PKA kinase activity. We also showed that Fyn induced the phosphorylation of cellular proteins within the PKA preferred target motif. ... [more]

Proteomes Sep. 29, 2018; 6(4); [Pubmed: 30274258]

Throughput

  • Low Throughput

Curated By

  • BioGRID