Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP)

UPS Project

GO Process (8)

GO Function (2)

GO Component (5)

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Protein interaction network revealed by quantitative proteomic analysis links TFIIB to multiple aspects of the transcription cycle.

O'Brien MJ, Ansari A

Although TFIIB is widely regarded as an initiation factor, recent reports have implicated it in multiple aspects of eukaryotic transcription. To investigate the broader role of TFIIB in transcription, we performed quantitative proteomic analysis of yeast TFIIB. We purified two different populations of TFIIB; one from soluble cell lysate, which is not engaged in transcription, and the other from the ... [more]

Biochim Biophys Acta Proteins Proteom Jan. 01, 2024; 1872(1);140968 [Pubmed: 37863410]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
UFD1 SUA7	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.1771	BioGRID	1934501

Curated By

BioGRID

Interaction Summary

SUA7

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core binding [IDA, IPI]
TBP-class protein binding [IDA, IPI]
TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA, IMP]
core RNA polymerase II recruiting transcription factor activity [IDA, IMP]

Gene Ontology Cellular Component

UFD1

Gene Ontology Biological Process

Gene Ontology Molecular Function

polyubiquitin binding [IDA]
ubiquitin binding [IDA]

Gene Ontology Cellular Component

Affinity Capture-MS

Publication

Protein interaction network revealed by quantitative proteomic analysis links TFIIB to multiple aspects of the transcription cycle.

Throughput

Related interactions

Curated By

Interaction Summary

SUA7

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core binding [IDA, IPI]TBP-class protein binding [IDA, IPI]TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA, IMP]core RNA polymerase II recruiting transcription factor activity [IDA, IMP]

Gene Ontology Cellular Component

UFD1

Gene Ontology Biological Process

Gene Ontology Molecular Function

polyubiquitin binding [IDA]ubiquitin binding [IDA]

Gene Ontology Cellular Component

Affinity Capture-MS

Publication

Protein interaction network revealed by quantitative proteomic analysis links TFIIB to multiple aspects of the transcription cycle.

Throughput

Related interactions

Curated By

RNA polymerase II core binding [IDA, IPI]
TBP-class protein binding [IDA, IPI]
TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA, IMP]
core RNA polymerase II recruiting transcription factor activity [IDA, IMP]

polyubiquitin binding [IDA]
ubiquitin binding [IDA]