Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans

UPS Project

GO Process (3)

GO Function (1)

GO Component (5)

Gene Ontology Biological Process

ER-associated ubiquitin-dependent protein catabolic process [IMP]

mitochondria-associated ubiquitin-dependent protein catabolic process [IMP]

nucleus-associated proteasomal ubiquitin-dependent protein catabolic process [IMP]

Gene Ontology Molecular Function

ubiquitin-specific protease activity [IDA]

Gene Ontology Cellular Component

Cdc48p-Npl4p-Vms1p AAA ATPase complex [IDA]

cytoplasm [IDA]

cytosol [IDA]

endoplasmic reticulum membrane [IDA]

mitochondrion [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Protein interaction network revealed by quantitative proteomic analysis links TFIIB to multiple aspects of the transcription cycle.

O'Brien MJ, Ansari A

Although TFIIB is widely regarded as an initiation factor, recent reports have implicated it in multiple aspects of eukaryotic transcription. To investigate the broader role of TFIIB in transcription, we performed quantitative proteomic analysis of yeast TFIIB. We purified two different populations of TFIIB; one from soluble cell lysate, which is not engaged in transcription, and the other from the ... [more]

Biochim Biophys Acta Proteins Proteom Jan. 01, 2024; 1872(1);140968 [Pubmed: 37863410]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
VMS1 SUA7	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.2313	BioGRID	2033870

Curated By

BioGRID

Interaction Summary

SUA7

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core binding [IDA, IPI]TBP-class protein binding [IDA, IPI]TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA, IMP]core RNA polymerase II recruiting transcription factor activity [IDA, IMP]

Gene Ontology Cellular Component

VMS1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ubiquitin-specific protease activity [IDA]

Gene Ontology Cellular Component

Affinity Capture-MS

Publication

Protein interaction network revealed by quantitative proteomic analysis links TFIIB to multiple aspects of the transcription cycle.

Throughput

Related interactions

Curated By

RNA polymerase II core binding [IDA, IPI]
TBP-class protein binding [IDA, IPI]
TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA, IMP]
core RNA polymerase II recruiting transcription factor activity [IDA, IMP]