FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p)

GO Process (4)

GO Function (2)

GO Component (3)

Gene Ontology Biological Process

NLS-bearing protein import into nucleus [IGI]

nuclear pore organization [IMP]

protein import into nucleus [IMP]

protein targeting to nuclear inner membrane [IGI]

Gene Ontology Molecular Function

nucleocytoplasmic transporter activity [IGI, IPI]
structural constituent of nuclear pore [IDA, IPI]

Gene Ontology Cellular Component

integral component of membrane [ISM]

nuclear pore [IDA]

nuclear pore central transport channel [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Protein interaction network revealed by quantitative proteomic analysis links TFIIB to multiple aspects of the transcription cycle.

O'Brien MJ, Ansari A

Although TFIIB is widely regarded as an initiation factor, recent reports have implicated it in multiple aspects of eukaryotic transcription. To investigate the broader role of TFIIB in transcription, we performed quantitative proteomic analysis of yeast TFIIB. We purified two different populations of TFIIB; one from soluble cell lysate, which is not engaged in transcription, and the other from the ... [more]

Biochim Biophys Acta Proteins Proteom Jan. 01, 2024; 1872(1);140968 [Pubmed: 37863410]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
NUP57 SUA7	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.1918	BioGRID	1935035

Curated By

BioGRID

Interaction Summary

SUA7

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core binding [IDA, IPI]TBP-class protein binding [IDA, IPI]TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA, IMP]core RNA polymerase II recruiting transcription factor activity [IDA, IMP]

Gene Ontology Cellular Component

NUP57

Gene Ontology Biological Process

Gene Ontology Molecular Function

nucleocytoplasmic transporter activity [IGI, IPI]structural constituent of nuclear pore [IDA, IPI]

Gene Ontology Cellular Component

Affinity Capture-MS

Publication

Protein interaction network revealed by quantitative proteomic analysis links TFIIB to multiple aspects of the transcription cycle.

Throughput

Related interactions

Curated By

RNA polymerase II core binding [IDA, IPI]
TBP-class protein binding [IDA, IPI]
TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA, IMP]
core RNA polymerase II recruiting transcription factor activity [IDA, IMP]

nucleocytoplasmic transporter activity [IGI, IPI]
structural constituent of nuclear pore [IDA, IPI]