BAIT
SUA7
SOH4, TFIIB, L000002141, YPR086W
Transcription factor TFIIB; a general transcription factor required for transcription initiation and start site selection by RNA polymerase II
GO Process (4)
GO Function (4)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
KTR7
L000004100, YIL085C
Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication
GO Process (2)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Protein interaction network revealed by quantitative proteomic analysis links TFIIB to multiple aspects of the transcription cycle.
Although TFIIB is widely regarded as an initiation factor, recent reports have implicated it in multiple aspects of eukaryotic transcription. To investigate the broader role of TFIIB in transcription, we performed quantitative proteomic analysis of yeast TFIIB. We purified two different populations of TFIIB; one from soluble cell lysate, which is not engaged in transcription, and the other from the ... [more]
Biochim Biophys Acta Proteins Proteom Jan. 01, 2024; 1872(1);140968 [Pubmed: 37863410]
Throughput
- High Throughput
Curated By
- BioGRID