An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Systematic identification of NF90 target RNAs by iCLIP analysis.

Lodde V, Floris M, Munk R, Martindale JL, Piredda D, Napodano CMP, Cucca F, Uzzau S, Abdelmohsen K, Gorospe M, Noh JH, Idda ML

RNA-binding proteins (RBPs) interact with and determine the fate of many cellular RNAs directing numerous essential roles in cellular physiology. Nuclear Factor 90 (NF90) is an RBP encoded by the interleukin enhancer-binding factor 3 (ILF3) gene that has been found to influence RNA metabolism at several levels, including pre-RNA splicing, mRNA turnover, and translation. To systematically identify the RNAs that ... [more]

Sci Rep Jan. 10, 2022; 12(1);364 [Pubmed: 35013429]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
IRF9 ILF3	Protein-RNA Protein-RNA An interaction is detected between and protein and an RNA in vitro.	Lodde V (2022)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

ILF3

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [IDA]RNA binding [NAS]double-stranded RNA binding [IDA]poly(A) RNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

IRF9