Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The ubiquitin-specific protease USP36 SUMOylates EXOSC10 and promotes the nucleolar RNA exosome function in rRNA processing.

Chen Y, Li Y, Dai RS, Savage JC, Shinde U, Klimek J, David LL, Young EA, Hafner M, Sears RC, Sun XX, Dai MS

The RNA exosome is an essential 3' to 5' exoribonuclease complex that mediates degradation, processing and quality control of virtually all eukaryotic RNAs. The nucleolar RNA exosome, consisting of a nine-subunit core and a distributive 3' to 5' exonuclease EXOSC10, plays a critical role in processing and degrading nucleolar RNAs, including pre-rRNA. However, how the RNA exosome is regulated in the ... [more]

Nucleic Acids Res May. 08, 2023; 51(8);3934-3949 [Pubmed: 36912080]

Throughput

  • Low Throughput

Curated By

  • BioGRID