BAIT

MCAM

CD146, MUC18
melanoma cell adhesion molecule
GO Process (4)
GO Function (0)
GO Component (3)
Homo sapiens
PREY

FYN

SLK, SYN, p59-FYN, RP1-66H14.1
FYN proto-oncogene, Src family tyrosine kinase
GO Process (29)
GO Function (6)
GO Component (5)
Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Cooperative polarization of MCAM/CD146 and ERM family proteins in melanoma.

Miller SG, Hoh M, Ebmeier CC, Tay JW, Ahn NG

The WRAMP structure is a protein network associated with tail-end actomyosin contractility, membrane retraction, and directional persistence during cell migration. A marker of WRAMP structures is melanoma cell adhesion molecule (MCAM) which dynamically polarizes to the cell rear. However, factors that mediate MCAM polarization are still unknown. In this study, BioID using MCAM as bait identifies the ERM family proteins, ... [more]

Mol Biol Cell Dec. 20, 2023; ();mbcE23060255 [Pubmed: 38117590]

Throughput

  • High Throughput

Additional Notes

  • BioID
  • Samples with a SAINT score corresponding to an FDR less than 0.05 were considered significant

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MCAM FYN
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID