An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Cooperative polarization of MCAM/CD146 and ERM family proteins in melanoma.

Miller SG, Hoh M, Ebmeier CC, Tay JW, Ahn NG

The WRAMP structure is a protein network associated with tail-end actomyosin contractility, membrane retraction, and directional persistence during cell migration. A marker of WRAMP structures is melanoma cell adhesion molecule (MCAM) which dynamically polarizes to the cell rear. However, factors that mediate MCAM polarization are still unknown. In this study, BioID using MCAM as bait identifies the ERM family proteins, ... [more]

Mol Biol Cell Dec. 20, 2023; ();mbcE23060255 [Pubmed: 38117590]

Throughput

High Throughput

Additional Notes

BioID
Samples with a SAINT score corresponding to an FDR less than 0.05 were considered significant

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RAB7A MCAM	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Yan BR (2021)	High	0	BioGRID	3378966

Curated By

BioGRID

Interaction Summary

MCAM

Gene Ontology Biological Process

Gene Ontology Cellular Component

RAB7A

Gene Ontology Biological Process

Gene Ontology Molecular Function

GDP binding [IDA]GTP binding [IDA]GTPase activity [IDA]protein binding [IPI]