BAIT
MCHR1
AW049955, Gpr24, Mch1r
melanin-concentrating hormone receptor 1
GO Process (5)
GO Function (3)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
RAB8A
AA409338, Mel
RAB8A, member RAS oncogene family
GO Process (15)
GO Function (6)
GO Component (21)
Gene Ontology Biological Process
- GTP catabolic process [IBA, ISO]
- Golgi vesicle fusion to target membrane [IBA, ISO]
- Rab protein signal transduction [IBA]
- axonogenesis [ISO]
- cellular response to insulin stimulus [IBA, ISO]
- cilium assembly [ISO]
- intracellular protein transport [IBA]
- protein localization to plasma membrane [IBA, ISO]
- protein secretion [IBA]
- regulation of exocytosis [IBA]
- regulation of long-term neuronal synaptic plasticity [ISO]
- regulation of protein localization [ISO]
- regulation of protein transport [ISO]
- synaptic vesicle exocytosis [IBA]
- vesicle docking involved in exocytosis [IBA, ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [ISO]
- centrosome [ISO]
- ciliary basal body [IDA]
- cilium [IDA, ISO]
- cytoplasmic membrane-bounded vesicle [ISO]
- dendrite [ISO]
- extracellular vesicular exosome [ISO]
- neuronal cell body [ISO]
- nonmotile primary cilium [IDA, ISO]
- nucleolus [ISO]
- nucleoplasm [ISO]
- nucleus [ISO]
- phagocytic vesicle [ISO]
- plasma membrane [IDA, ISO]
- postsynaptic density [ISO]
- primary cilium [ISO]
- recycling endosome [IDA]
- recycling endosome membrane [ISO]
- secretory granule membrane [IBA]
- synaptic vesicle [IBA]
- trans-Golgi network transport vesicle [IDA]
Mus musculus
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Identification of new ciliary signaling pathways in the brain and insights into neurological disorders.
Primary cilia are conserved sensory hubs essential for signaling transduction and embryonic development. Ciliary dysfunction causes a variety of developmental syndromes with neurological features and cognitive impairment, whose basis mostly remains unknown. Despite connections to neural function, the primary cilium remains an overlooked organelle in the brain. Most neurons have a primary cilium; however, it is still unclear how this ... [more]
bioRxiv Dec. 21, 2023; (); [Pubmed: 38187761]
Throughput
- High Throughput
Additional Notes
- BioID
- Proteins were considered hits if they showed at least a 2-fold enrichment in GPCRs-BirA2 compared to BirA2 alone, and a false discovery rate of at least 0.7 (p-value of 0.036 or lower)
Curated By
- BioGRID