Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Identification of new ciliary signaling pathways in the brain and insights into neurological disorders.

Loukil A, Ebright E, Uezu A, Gao Y, Soderling SH, Goetz SC

Primary cilia are conserved sensory hubs essential for signaling transduction and embryonic development. Ciliary dysfunction causes a variety of developmental syndromes with neurological features and cognitive impairment, whose basis mostly remains unknown. Despite connections to neural function, the primary cilium remains an overlooked organelle in the brain. Most neurons have a primary cilium; however, it is still unclear how this ... [more]

bioRxiv Dec. 21, 2023; (); [Pubmed: 38187761]

Throughput

  • High Throughput

Additional Notes

  • BioID
  • Proteins were considered hits if they showed at least a 2-fold enrichment in GPCRs-BirA2 compared to BirA alone, and a false discovery rate of at least 0.7 (p-value of 0.036 or lower)

Curated By

  • BioGRID