BAIT

NFE2L2

AI194320, Nrf2, RP23-374O4.2
nuclear factor, erythroid derived 2, like 2
GO Process (24)
GO Function (10)
GO Component (7)
Mus musculus
PREY

NEDD4

AA959633, AL023035, AU019897, E430025J12Rik, Nedd4-1, Nedd4a, mKIAA0093
neural precursor cell expressed, developmentally down-regulated 4
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Celastrol targeting Nedd4 reduces Nrf2-mediated oxidative stress in astrocytes after ischemic stroke.

Hong Z, Cao J, Liu D, Liu M, Chen M, Zeng F, Qin Z, Wang J, Tao T

Stroke is the second leading cause of death worldwide, and oxidative stress plays a crucial role. Celastrol exhibits strong antioxidant properties in several diseases; however, whether it can affect oxidation in cerebral ischemic-reperfusion injury (CIRI) remains unclear. This study aimed to determine whether celastrol could reduce oxidative damage during CIRI and to elucidate the underlying mechanisms. Here, we found that ... [more]

J Pharm Anal Feb. 01, 2023; 13(2);156-169 [Pubmed: 36908855]

Throughput

  • Low Throughput

Curated By

  • BioGRID