BAIT
UBE2H
E2-20K, GID3, UBC8, UBCH, UBCH2
ubiquitin-conjugating enzyme E2H
GO Process (3)
GO Function (5)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
AP2M1
AP50, CLAPM1, mu2
adaptor-related protein complex 2, mu 1 subunit
GO Process (11)
GO Function (5)
GO Component (7)
Gene Ontology Biological Process
- antigen processing and presentation of exogenous peptide antigen via MHC class II [TAS]
- axon guidance [TAS]
- clathrin-mediated endocytosis [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- membrane organization [TAS]
- negative regulation of epidermal growth factor receptor signaling pathway [TAS]
- negative regulation of protein localization to plasma membrane [IMP]
- neurotrophin TRK receptor signaling pathway [TAS]
- regulation of defense response to virus by virus [TAS]
- synaptic transmission [TAS]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Multisite phosphorylation dictates selective E2-E3 pairing as revealed by Ubc8/UBE2H-GID/CTLH assemblies.
Ubiquitylation is catalyzed by coordinated actions of E3 and E2 enzymes. Molecular principles governing many important E3-E2 partnerships remain unknown, including those for RING-family GID/CTLH E3 ubiquitin ligases and their dedicated E2, Ubc8/UBE2H (yeast/human nomenclature). GID/CTLH-Ubc8/UBE2H-mediated ubiquitylation regulates biological processes ranging from yeast metabolic signaling to human development. Here, cryoelectron microscopy (cryo-EM), biochemistry, and cell biology reveal this exquisitely specific ... [more]
Mol Cell Jan. 18, 2024; 84(2);293-308.e14 [Pubmed: 38113892]
Throughput
- High Throughput
Curated By
- BioGRID