BAIT
EFNA1
B61, ECKLG, EFL1, EPLG1, LERK-1, LERK1, TNFAIP4
ephrin-A1
GO Process (15)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
- angiogenesis [IBA]
- aortic valve morphogenesis [ISS]
- axon guidance [IBA]
- cell migration [IDA]
- cell-cell signaling [TAS]
- endocardial cushion to mesenchymal transition involved in heart valve formation [ISS]
- ephrin receptor signaling pathway [IDA]
- mitral valve morphogenesis [ISS]
- negative regulation of dendritic spine morphogenesis [ISS]
- negative regulation of epithelial to mesenchymal transition [ISS]
- negative regulation of transcription from RNA polymerase II promoter [ISS]
- positive regulation of peptidyl-tyrosine phosphorylation [IDA]
- regulation of cell adhesion mediated by integrin [IDA]
- regulation of peptidyl-tyrosine phosphorylation [ISS]
- substrate adhesion-dependent cell spreading [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ACTB
BRWS1, PS1TP5BP1
actin, beta
GO Process (17)
GO Function (8)
GO Component (15)
Gene Ontology Biological Process
- 'de novo' posttranslational protein folding [TAS]
- ATP-dependent chromatin remodeling [IDA]
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- adherens junction organization [TAS]
- axon guidance [TAS]
- blood coagulation [TAS]
- cell junction assembly [TAS]
- cell-cell junction organization [TAS]
- cellular component movement [TAS]
- cellular protein metabolic process [TAS]
- chromatin organization [TAS]
- innate immune response [TAS]
- membrane organization [TAS]
- platelet aggregation [IMP]
- protein folding [TAS]
- retina homeostasis [IEP]
- substantia nigra development [IEP]
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- Tat protein binding [IPI]
- kinesin binding [IPI]
- nitric-oxide synthase binding [IPI]
- nucleosomal DNA binding [IDA]
- protein binding [IPI]
- structural constituent of cytoskeleton [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- Tat protein binding [IPI]
- kinesin binding [IPI]
- nitric-oxide synthase binding [IPI]
- nucleosomal DNA binding [IDA]
- protein binding [IPI]
- structural constituent of cytoskeleton [TAS]
Gene Ontology Cellular Component
- MLL5-L complex [IDA]
- NuA4 histone acetyltransferase complex [IDA]
- blood microparticle [IDA]
- cytoplasm [TAS]
- cytoplasmic ribonucleoprotein granule [IDA]
- cytoskeleton [TAS]
- cytosol [TAS]
- extracellular space [IDA]
- extracellular vesicular exosome [IDA]
- focal adhesion [IDA]
- membrane [IDA]
- nuclear chromatin [IDA]
- nucleoplasm [TAS]
- protein complex [IDA]
- ribonucleoprotein complex [IDA]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Ephrin-As, Eph receptors and integrin ?3 interact and colocalise at membrane protrusions of U251MG glioblastoma cells.
Glioblastoma is the most common brain cancer. Ephrins and their Eph receptors play important roles in the development of central nervous system and the regulation of cancer cell migration and invasion. In a search for the Eph receptor complexes, we used tandem affinity purification based interaction screening with tagged ephrins A1, A3 and A4 combined with protein identification by mass-spectrometry ... [more]
Cell Biol Int Oct. 01, 2013; 37(10);1080-8 [Pubmed: 23686814]
Throughput
- Low Throughput
Curated By
- BioGRID