Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Ephrin-As, Eph receptors and integrin ?3 interact and colocalise at membrane protrusions of U251MG glioblastoma cells.

Makarov A, Ylivinkka I, Nyman TA, Hyytiaeinen M, Keski-Oja J

Glioblastoma is the most common brain cancer. Ephrins and their Eph receptors play important roles in the development of central nervous system and the regulation of cancer cell migration and invasion. In a search for the Eph receptor complexes, we used tandem affinity purification based interaction screening with tagged ephrins A1, A3 and A4 combined with protein identification by mass-spectrometry ... [more]

Cell Biol Int Oct. 01, 2013; 37(10);1080-8 [Pubmed: 23686814]

Throughput

  • Low Throughput

Curated By

  • BioGRID