Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The negative effect of G1958A polymorphism on MTHFD1 protein stability and HCC growth.

Rao K, Zheng K, Zhao Q, He J, Zhou B, Hou G, Sha N, Wang W, Yan M, Zhou Y, Jin Y, Jiang Y, Xia Q

Methylenetetrahydrofolate dehydrogenase (MTHFD1), a key enzyme on the folate pathway, has been implicated in the tumor development of distinct types of cancers. The single nucleotide polymorphism (SNP) of 1958G?>?A mutation in the coding region of MTHFD1 (arginine 653 is mutated into glutamine) has been detected in a significant proportion of clinical samples of hepatocellular carcinoma (HCC). METHODS?: Hepatoma cell lines, ... [more]

Cell Oncol (Dordr) Jun. 01, 2023; 46(3);735-744 [Pubmed: 36913067]

Throughput

  • High Throughput

Additional Notes

  • Affinity capture MS was carried out to identify high confidence protein interactors for the G1958A SNP of MTHFD1 (R653Q)

Curated By

  • BioGRID