BAIT
FBXL4
FBL4, FBL5, MTDPS13
F-box and leucine-rich repeat protein 4
GO Process (1)
GO Function (0)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Homo sapiens
PREY
DNAJA1
DJ-2, DjA1, HDJ2, HSDJ, HSJ2, HSPF4, NEDD7, hDJ-2, RP11-54K16.1
DnaJ (Hsp40) homolog, subfamily A, member 1
GO Process (9)
GO Function (7)
GO Component (5)
Gene Ontology Biological Process
- negative regulation of JUN kinase activity [IMP]
- negative regulation of apoptotic process [IMP]
- negative regulation of protein ubiquitination [IDA]
- positive regulation of apoptotic process [IMP]
- protein folding [TAS]
- protein localization to mitochondrion [IMP]
- protein refolding [IBA]
- regulation of protein transport [IDA]
- response to unfolded protein [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A mitochondrial SCF-FBXL4 ubiquitin E3 ligase complex degrades BNIP3 and NIX to restrain mitophagy and prevent mitochondrial disease.
Mitophagy is a fundamental quality control mechanism of mitochondria. Its regulatory mechanisms and pathological implications remain poorly understood. Here, via a mitochondria-targeted genetic screen, we found that knockout (KO) of FBXL4, a mitochondrial disease gene, hyperactivates mitophagy at basal conditions. Subsequent counter screen revealed that FBXL4-KO hyperactivates mitophagy via two mitophagy receptors BNIP3 and NIX. We determined that FBXL4 functions ... [more]
EMBO J Jul. 03, 2023; 42(13);e113033 [Pubmed: 36896912]
Throughput
- High Throughput
Curated By
- BioGRID