Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

TOR-mediated Ypt1 phosphorylation regulates autophagy initiation complex assembly.

Yao W, Chen Y, Chen Y, Zhao P, Liu J, Zhang Y, Jiang Q, Wu C, Xie Y, Fan S, Ye M, Wang Y, Feng Y, Bai X, Fan M, Feng S, Wang J, Cui Y, Xia H, Ma C, Xie Z, Zhang L, Sun Q, Liu W, Yi C

The regulation of autophagy initiation is a key step in autophagosome biogenesis. However, our understanding of the molecular mechanisms underlying the stepwise assembly of ATG proteins during this process remains incomplete. The Rab GTPase Ypt1/Rab1 is recognized as an essential autophagy regulator. Here, we identify Atg23 and Atg17 as binding partners of Ypt1, with their direct interaction proving crucial for ... [more]

EMBO J Oct. 04, 2023; 42(19);e112814 [Pubmed: 37635626]

Throughput

  • Low Throughput

Curated By

  • BioGRID