BAIT

FBXL8

FBL8
F-box and leucine-rich repeat protein 8
UPS Project
GO Process (0)
GO Function (1)
GO Component (0)

Gene Ontology Molecular Function

Homo sapiens
PREY

RBX1

BA554C12.1, RNF75, ROC1, RP11-554C12.1
ring-box 1, E3 ubiquitin protein ligase
Cell Cycle - Senescence Project
Fanconi Anemia Project
UPS Project
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

SCF-FBXL8 contributes to liver metastasis and stem-cell-like features in colorectal cancer cells by mediating ubiquitination and degradation of TP53.

Yao J, Wang XP, Yang J, Yang Z, Zhang ZY

FBXL8 is a conserved F-box protein, belonging to the ubiquitin ligase complex, which promotes the development and progression of tumours. However, the regulation function and mechanism of FBXL8's involvement in colorectal cancer (CRC) remain unclear.RT-PCR is used to detect gene expression levels. Protein levels were determined by western blotting and flow cytometry. The bindings of FBXL8 and p53 and ubiquitination ... [more]

Clin Transl Med Mar. 01, 2023; 13(3);e1208 [Pubmed: 36855778]

Throughput

  • Low Throughput

Curated By

  • BioGRID