An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

SARS-CoV-2 Nsp6 damages Drosophila heart and mouse cardiomyocytes through MGA/MAX complex-mediated increased glycolysis.

Zhu JY, Wang G, Huang X, Lee H, Lee JG, Yang P, van de Leemput J, Huang W, Kane MA, Yang P, Han Z

SARS-CoV-2 infection causes COVID-19, a severe acute respiratory disease associated with cardiovascular complications including long-term outcomes. The presence of virus in cardiac tissue of patients with COVID-19 suggests this is a direct, rather than secondary, effect of infection. Here, by expressing individual SARS-CoV-2 proteins in the Drosophila heart, we demonstrate interaction of virus Nsp6 with host proteins of the MGA/MAX ... [more]

Commun Biol Sep. 30, 2022; 5(1);1039 [Pubmed: 36180527]

Throughput

High Throughput

Additional Notes

HEK 293T cells

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
NSP6 ADRM1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Broderick K (2025)	High	-	BioGRID	3801899

Curated By

BioGRID

Interaction Summary

NSP6

ADRM1

Gene Ontology Biological Process

Gene Ontology Molecular Function

endopeptidase activator activity [IDA]protease binding [IPI]proteasome binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

Affinity Capture-MS