An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Interaction network of human early embryonic transcription factors.

Gawriyski L, Tan Z, Liu X, Chowdhury I, Malaymar Pinar D, Zhang Q, Weltner J, Jouhilahti EM, Wei GH, Kere J, Varjosalo M

Embryonic genome activation (EGA) occurs during preimplantation development and is characterized by the initiation of de novo transcription from the embryonic genome. Despite its importance, the regulation of EGA and the transcription factors involved in this process are poorly understood. Paired-like homeobox (PRDL) family proteins are implicated as potential transcriptional regulators of EGA, yet the PRDL-mediated gene regulatory networks remain ... [more]

EMBO Rep Jan. 31, 2024; (); [Pubmed: 38297188]

Throughput

High Throughput

Additional Notes

BioID
Statistically significant (BFDR less than 0.01) and contaminant filtered BioID-MS data

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
KLF3 CTBP1	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Goos H (2022)	High	-	BioGRID	3315930
CTBP1 KLF3	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Corominas R (2014)	High	-	BioGRID	-
KLF3 CTBP1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Yachie N (2016)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

KLF3

Gene Ontology Biological Process

Gene Ontology Molecular Function

sequence-specific DNA binding transcription factor activity [NAS]

CTBP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

NAD binding [ISS]RNA polymerase II transcription corepressor activity [IDA]protein C-terminus binding [TAS]protein binding [IPI]protein domain specific binding [IDA, IPI]repressing transcription factor binding [IPI]transcription factor binding [IPI]

Gene Ontology Cellular Component

Proximity Label-MS