Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

TBK1 phosphorylation activates LIR-dependent degradation of the inflammation repressor TNIP1.

Zhou J, Rasmussen NL, Olsvik HL, Akimov V, Hu Z, Evjen G, Kaeser-Pebernard S, Sankar DS, Roubaty C, Verlhac P, van de Beek N, Reggiori F, Abudu YP, Blagoev B, Lamark T, Johansen T, Dengjel J

Limitation of excessive inflammation due to selective degradation of pro-inflammatory proteins is one of the cytoprotective functions attributed to autophagy. In the current study, we highlight that selective autophagy also plays a vital role in promoting the establishment of a robust inflammatory response. Under inflammatory conditions, here TLR3-activation by poly(I:C) treatment, the inflammation repressor TNIP1 (TNFAIP3 interacting protein 1) is ... [more]

J Cell Biol Feb. 06, 2023; 222(2); [Pubmed: 36574265]

Throughput

  • Low Throughput

Curated By

  • BioGRID