BAIT

TRRAP

PAF350/400, PAF400, STAF40, TR-AP, Tra1
transformation/transcription domain-associated protein
Glioblastoma Project
Homo sapiens
PREY

RHOA

ARH12, ARHA, RHO12, RHOH12
ras homolog family member A
Human Papilloma Virus (HPV) Project
GO Process (29)
GO Function (3)
GO Component (8)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

HSF1 phosphorylation establishes an active chromatin state via the TRRAP-TIP60 complex and promotes tumorigenesis.

Fujimoto M, Takii R, Matsumoto M, Okada M, Nakayama KI, Nakato R, Fujiki K, Shirahige K, Nakai A

Transcriptional regulation by RNA polymerase II is associated with changes in chromatin structure. Activated and promoter-bound heat shock transcription factor 1 (HSF1) recruits transcriptional co-activators, including histone-modifying enzymes; however, the mechanisms underlying chromatin opening remain unclear. Here, we demonstrate that HSF1 recruits the TRRAP-TIP60 acetyltransferase complex in HSP72 promoter during heat shock in a manner dependent on phosphorylation of HSF1-S419. ... [more]

Nat Commun Dec. 29, 2021; 13(1);4355 [Pubmed: 35906200]

Throughput

  • High Throughput

Additional Notes

  • Affinity capture MS was carried out to identify high confidence protein interactors enriched upon heat shock

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RHOA TRRAP
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
3331818

Curated By

  • BioGRID