BAIT

ASH2L

ASH2, ASH2L1, ASH2L2, Bre2
ash2 (absent, small, or homeotic)-like (Drosophila)
Homo sapiens
PREY

KPNA2

IPOA1, QIP2, RCH1, SRP1alpha
karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Epigenetic-focused CRISPR/Cas9 screen identifies (absent, small, or homeotic)2-like protein (ASH2L) as a regulator of glioblastoma cell survival.

Ozyerli-Goknar E, Kala EY, Aksu AC, Bulut I, Cingoez A, Nizamuddin S, Biniossek M, Seker-Polat F, Morova T, Aztekin C, Kung SHY, Syed H, Tuncbag N, Goenen M, Philpott M, Cribbs AP, Acilan C, Lack NA, Onder TT, Timmers HTM, Bagci-Onder T

Glioblastoma is the most common and aggressive primary brain tumor with extremely poor prognosis, highlighting an urgent need for developing novel treatment options. Identifying epigenetic vulnerabilities of cancer cells can provide excellent therapeutic intervention points for various types of cancers.In this study, we investigated epigenetic regulators of glioblastoma cell survival through CRISPR/Cas9 based genetic ablation screens using a customized sgRNA ... [more]

Cell Commun Signal Nov. 16, 2023; 21(1);328 [Pubmed: 37974198]

Throughput

  • High Throughput

Additional Notes

  • U373 cells cytoplasm fraction
  • U87MG cells cytoplasm fraction

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ASH2L KPNA2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-

Curated By

  • BioGRID