An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

SARS-CoV-2 nucleocapsid protein binds host mRNAs and attenuates stress granules to impair host stress response.

Nabeel-Shah S, Lee H, Ahmed N, Burke GL, Farhangmehr S, Ashraf K, Pu S, Braunschweig U, Zhong G, Wei H, Tang H, Yang J, Marcon E, Blencowe BJ, Zhang Z, Greenblatt JF

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid (N) protein is essential for viral replication, making it a promising target for antiviral drug and vaccine development. SARS-CoV-2 infected patients exhibit an uncoordinated immune response; however, the underlying mechanistic details of this imbalance remain obscure. Here, starting from a functional proteomics workflow, we cataloged the protein-protein interactions of SARS-CoV-2 proteins, including ... [more]

iScience Jan. 21, 2022; 25(1);103562 [Pubmed: 34901782]

Throughput

High Throughput

Additional Notes

Affinity capture MS was carried out to identify high confidence protein interactors with BFDR of less than or equal to 0.01

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
G3BP1 FXR1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Jain S (2016)	High	-	BioGRID	-
G3BP1 FXR1	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Youn JY (2018)	Low	-	BioGRID	2817992
FXR1 G3BP1	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Youn JY (2018)	High	-	BioGRID	2813913

Curated By

BioGRID

Interaction Summary

G3BP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP-dependent DNA helicase activity [TAS]ATP-dependent RNA helicase activity [TAS]poly(A) RNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

FXR1

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA binding [TAS]mRNA binding [IBA]poly(A) RNA binding [IDA]