An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

SARS-CoV-2 nucleocapsid protein binds host mRNAs and attenuates stress granules to impair host stress response.

Nabeel-Shah S, Lee H, Ahmed N, Burke GL, Farhangmehr S, Ashraf K, Pu S, Braunschweig U, Zhong G, Wei H, Tang H, Yang J, Marcon E, Blencowe BJ, Zhang Z, Greenblatt JF

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid (N) protein is essential for viral replication, making it a promising target for antiviral drug and vaccine development. SARS-CoV-2 infected patients exhibit an uncoordinated immune response; however, the underlying mechanistic details of this imbalance remain obscure. Here, starting from a functional proteomics workflow, we cataloged the protein-protein interactions of SARS-CoV-2 proteins, including ... [more]

iScience Jan. 21, 2022; 25(1);103562 [Pubmed: 34901782]

Throughput

High Throughput

Additional Notes

Affinity capture MS was carried out to identify high confidence protein interactors for full length N protein, in cells under oxidative stress, with BFDR of less than or equal to 0.01
Affinity capture MS was carried out to identify high confidence protein interactors for full length N protein, in nonstressed cells, with BFDR of less than or equal to 0.01

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
N MYH10	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Chen X (2024)	High	-	BioGRID	3753651
N MYH10	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Ren J (2024)	High	-	BioGRID	-
N MYH10	Affinity Capture-RNA Affinity Capture-RNA An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.	Whitworth IT (2023)	High	-	BioGRID	3559078
N MYH10	Affinity Capture-RNA Affinity Capture-RNA An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.	Whitworth IT (2024)	High	-	BioGRID	3777870

Curated By

BioGRID

Interaction Summary

N

MYH10

Gene Ontology Biological Process

Gene Ontology Molecular Function

ADP binding [IDA]ATP binding [IDA, NAS]actin binding [NAS]actin filament binding [IDA, IMP]actin-dependent ATPase activity [IDA, IMP]microfilament motor activity [IDA]protein binding [IPI]

Gene Ontology Cellular Component

Affinity Capture-MS