BAIT

RAF1

CMD1NN, CRAF, NS5, Raf-1, c-Raf
Raf-1 proto-oncogene, serine/threonine kinase
GO Process (30)
GO Function (5)
GO Component (5)
Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Mitochondrial Raf1 Regulates Glutamine Catabolism.

Shanderson RL, Ferguson ID, Siprashvili Z, Ducoli L, Li AM, Miao W, Srinivasan S, Velasco MG, Li Y, Ye J, Khavari P

Raf1 is present within the mitochondrial matrix, where it binds GLS to regulate glutamine catabolism and tumorigenesis. In cancer, Raf1 activation occurs via mechanisms that include mutation of upstream regulators, such as receptor tyrosine kinases and Ras GTPases, as well as by mutations that affect RAF1 itself, including via gene amplification ( 1 - 4 ). Once recruited to the ... [more]

bioRxiv Mar. 09, 2024; (); [Pubmed: 38496616]

Throughput

  • High Throughput

Additional Notes

  • BioID
  • Raf1 proximal protein with SAINT greater or equal to 0.9 in melanoma cell lines

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RAF1 LATS2
FRET
FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

High-BioGRID
2641233

Curated By

  • BioGRID