An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

HIV-1 Gag co-opts a cellular complex containing DDX6, a helicase that facilitates capsid assembly.

Reed JC, Molter B, Geary CD, McNevin J, McElrath J, Giri S, Klein KC, Lingappa JR

To produce progeny virus, human immunodeficiency virus type I (HIV-1) Gag assembles into capsids that package the viral genome and bud from the infected cell. During assembly of immature capsids, Gag traffics through a pathway of assembly intermediates (AIs) that contain the cellular adenosine triphosphatase ABCE1 (ATP-binding cassette protein E1). In this paper, we showed by coimmunoprecipitation and immunoelectron microscopy ... [more]

J Cell Biol Aug. 06, 2012; 198(3);439-56 [Pubmed: 22851315]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
DCP2 ABCE1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Li S (2014)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

DCP2

Gene Ontology Biological Process

Gene Ontology Molecular Function

exoribonuclease activity, producing 5'-phosphomonoesters [TAS]m7G(5')pppN diphosphatase activity [IDA]protein binding [IPI]

Gene Ontology Cellular Component

ABCE1