An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Tau interactome maps synaptic and mitochondrial processes associated with neurodegeneration.

Tracy TE, Madero-Perez J, Swaney DL, Chang TS, Moritz M, Konrad C, Ward ME, Stevenson E, Huettenhain R, Kauwe G, Mercedes M, Sweetland-Martin L, Chen X, Mok SA, Wong MY, Telpoukhovskaia M, Min SW, Wang C, Sohn PD, Martin J, Zhou Y, Luo W, Trojanowski JQ, Lee VMY, Gong S, Manfredi G, Coppola G, Krogan NJ, Geschwind DH, Gan L

Tau (MAPT) drives neuronal dysfunction in Alzheimer disease (AD) and other tauopathies. To dissect the underlying mechanisms, we combined an engineered ascorbic acid peroxidase (APEX) approach with quantitative affinity purification mass spectrometry (AP-MS) followed by proximity ligation assay (PLA) to characterize Tau interactomes modified by neuronal activity and mutations that cause frontotemporal dementia (FTD) in human induced pluripotent stem cell ... [more]

Cell Feb. 17, 2022; 185(4);712-728.e14 [Pubmed: 35063084]

Throughput

High Throughput

Additional Notes

N-APEX tagged Tau
ascorbic acid peroxidase (APEX)-tagged Tau approach followed by quantitative affinity purification mass spectrometry (AP-MS) followed by proximity ligation assay (PLA)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PPP1CC MAPT	Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.	Liu F (2005)	Low	-	BioGRID	2623981

Curated By

BioGRID

Interaction Summary

MAPT

Gene Ontology Biological Process

Gene Ontology Molecular Function

SH3 domain binding [IPI]apolipoprotein binding [IPI]enzyme binding [IPI]lipoprotein particle binding [IPI]microtubule binding [IDA]protein binding [IPI]structural constituent of cytoskeleton [TAS]

Gene Ontology Cellular Component

PPP1CC

Gene Ontology Biological Process

Gene Ontology Molecular Function

phosphatase activity [ISS]phosphoprotein phosphatase activity [TAS]poly(A) RNA binding [IDA]protein binding [IPI]protein kinase binding [IPI]protein serine/threonine phosphatase activity [IMP]